Abstract
We investigated the function and expression pattern of the transient receptor potential
melastatin-8 (TRPM8) in urinary bladder afferent neurons from control and bladder
outlet obstruction (BOO) rats. BOO was produced and, after six weeks, the effects
of intravesical infusion of menthol, the agonist of TRPM8, were investigated using
unanesthetized cystometry. The intravesical infusion of menthol produced an increase
in the micturition pressure in both sham surgery and BOO rats. In BOO rats, increased
basal and threshold pressure and a decreased micturition interval were observed. Next,
the population of TRPM8-positive and the co-expression proportion of TRPM8 with neurochemical
markers (NF200 or TRPV1) in the bladder afferent neurons were each compared between
the control and BOO rats using retrograde tracing and immunohistochemistry. The population
of TRPM8-immunoreactive bladder afferent neurons was larger in BOO rats (3.28±0.43%) than in the control rats (1.33±0.18%). However, there were no statistical differences between the control and BOO
rats in the co-expression proportion of neither TRPM8-NF200 (84.1±4.3% vs 79.7±2.7%, p=0.41) nor TRPM8-TRPV1 (33.3±3.6% vs 40.8±2.6%, p=0.08) in the bladder afferent neurons. The present results suggest that the neuronal
input through TRPM8-positive bladder afferent neurons are augmented after BOO, however,
the neurochemical phenotype of the up-regulated TRPM8-positive bladder afferent neurons
is not changed after BOO.
Keywords
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References
- TRPM8 protein localization in trigeminal ganglion and taste papillae.Brain Res. Mol. Brain Res. 2005; 136: 91-98
- Detection of cold pain, cold allodynia and cold hyperalgesia in freely behaving rats.Mol. Pain. 2005; 1: 36
- Two populations of cold-sensitive neurons in rat dorsal root ganglia and their modulation by nerve growth factor.Eur. J. Neurosci. 2004; 20: 2276-2282
- Mechanisms of disease: involvement of the urothelium in bladder dysfunction.Nature Clin. Pract. Urol. 2007; 4: 46-54
- Activation of urothelial transient receptor potential vanilloid 4 by 4α-phorbol 12,13-didecanoate contributes to altered bladder reflexes in the rat.J. Pharmacol. Exp. Ther. 2007; 323: 227-235
- Spinal reflex activity from the vesical mucosa in paraplegic patients.AMA Arch. Neurol. Psychiatry. 1957; 160: 339-354
- The contribution of TRPM8 and TRPA1 channels to cold allodynia and neuropathic pain.PloS One. 2009; 4: e7383
- The incidence of a positive ice water test in bladder outlet obstructed patients: evidence for bladder neural plasticity.J. Urol. 1998; 160: 34-38
- A shifted paradigm for the further understanding, evaluation, and treatment of lower urinary tract symptoms in men: focus on the bladder.Eur. Urol. 2006; 49: 651-658
- Detrusor-sphincter dyssynergia induced by cold stimulation of the urinary bladder of rats.Am. J. Physiol. 1997; 272: R1271-R1282
- TRP channel knockout mice lose their cool.Neuron. 2007; 54: 345-347
- Attenuated cold sensitivity in TRPM8 null mice.Neuron. 2007; 54: 379-386
- Intravesical capsaicin in neurologic impaired patients with detrusor hyperreflexia.J. Spinal Cord Med. 1996; 19: 190-193
- Transient receptor potential channel A1 involved in sensory transduction of rat urinary bladder through C-fiber pathway.Urology. 2007; 70: 826-831
- Differential expression profile of cold (TRPA1) and cool (TRPM8) receptors in human urogenital organs.Urology. 2008; 72: 450-455
- A bladder-to-bladder cooling reflex in the cat.J. Physiol. 1990; 427: 281-300
- Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury.Biochem. Biophys. Res. Commun. 2007; 358: 1058-1064
- Evidence of cold receptors in the human bladder: effect of menthol on the bladder cooling reflex.J. Urol. 1993; 150: 427-430
- The bladder cooling reflex and the use of cooling as stimulus to the lower urinary tract.J. Urol. 1999; 162: 1890-1896
- Positive bladder cooling reflex in patients with bladder outlet obstruction due to benign prostatic hyperplasia.World J. Urol. 1999; 17: 126-130
- Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder.Neurosci. Res. 2009; 65: 245-251
- NGF involvement in pain induced by chronic constriction injury of the rat sciatic nerve.NeuroReport. 1997; 8: 1613-1618
- Positive response to ice water test associated with high-grade bladder outlet obstruction in patients with benign prostatic hyperplasia.Urology. 2003; 62: 909-913
- Cold- and menthol-sensitive C afferents of cat urinary bladder.J. Physiol. 2002; 543: 211-220
- Bimodal action of menthol on the transient receptor potential channel TRPA1.J. Neurosci. 2007; 27: 9874-9884
- Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with A δ/C-fibers and colocalization with Trk receptors.J. Comp. Neurol. 2005; 493: 596-606
- Functional TRP and ASIC-like channels in cultured urothelial cells from the rat.Am. J. Physiol. Renal Physiol. 2009; 296: F892-F901
- AMTB, a TRPM8 channel blocker: evidence in rats for activity in overactive bladder and painful bladder syndrome.Am. J. Physiol. Renal Physiol. 2008; 295: F803-F810
- Effect of menthol on the bladder cooling reflex in the cat.Acta Physiol. Scand. 1991; 141: 1-10
- Inhibition of the bladder cooling reflex in the awake state: an experimental study in the cat.J. Urol. 2004; 172: 2051-2053
- More than cool: promiscuous relationships of menthol and other sensory compounds.Mol. Cell. Neurosci. 2006; 32: 335-343
- Changes in contractile properties in hypertrophic rat urinary bladder.J. Urol. 1982; 128: 1340-1342
- The C fibre reflex of the cat urinary bladder.J. Physiol. 1998; 513: 531-541
- Identification of a cold receptor reveals a general role for TRP channels in thermosensation.Nature. 2002; 416: 52-58
- Cool and menthol receptor TRPM8 in human urinary bladder disorders and clinical correlations.BMC Urol. 2006; 6: 6
- Role of TRPM8 and TRPA1 for cold allodynia in patients with cold injury.Pain. 2008; 139: 63-72
- Effect of menthol on detrusor smooth-muscle contraction and the micturition reflex in rats.Urology. 2008; 72: 701-705
- A TRP channel that senses cold stimuli and menthol.Cell. 2002; 108: 705-715
- Analgesia mediated by the TRPM8 cold receptor in chronic neuropathic pain.Curr. Biol. 2006; 16: 1591-1605
- A cold- and menthol-activated current in rat dorsal root ganglion neurones: properties and role in cold transduction.J. Physiol. 2002; 545: 595-614
- The effect of experimental urethral obstruction and its reversal on changes in passive electrical properties of detrusor muscle.J. Urol. 1992; 148: 1957-1961
- Bladder outflow obstruction—a cause of denervation supersensitivity.J. Urol. 1987; 138: 1461-1466
- Effect of bladder outlet obstruction on micturition reflex pathways in the rat.J. Urol. 1988; 140: 864-871
- Nerve growth factor in the urinary bladder of the adult regulates neuronal form and function.J. Clin. Invest. 1991; 88: 1709-1715
- Immunity to nerve growth factor prevents afferent plasticity following urinary bladder hypertrophy.J. Urol. 1996; 155: 379-385
- Cool (TRPM8) and hot (TRPV1) receptors in the bladder and male genital tract.J. Urol. 2004; 172: 1175-1178
- ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures.Cell. 2003; 112: 819-829
- Effect of preemptive treatment of capsaicin or resiniferatoxin on the development of pre-micturition contractions after partial urethral obstruction in the rat.J. Urol. 2003; 170: 1022-1026
- Cold response of the bladder in guinea pig: involvement of transient receptor potential channel, TRPM8.Urology. 2005; 65: 406-410
- Chemical and cold sensitivity of two distinct populations of TRPM8-expressing somatosensory neurons.J. Neurophysiol. 2006; 95: 1221-1230
Article info
Publication history
Published online: June 20, 2011
Accepted:
May 21,
2011
Received in revised form:
May 3,
2011
Received:
November 26,
2010
Identification
Copyright
© 2011 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
