Several reports have shown that changes in autonomic receptor expression during disease
can influence the contractile response (1,2). The present study aimed to characterize
how activation of autonomic receptors affects the proliferation rate of a normal and
a malignant urothelial cell line, designated UROtsa and T24, respectively. Both cell
lines were treated with a muscarinic, adrenergic or nicotinic receptor agonist. Neither
the muscarinic agonist methacholine nor nicotine affected the proliferation rate of
any of the two cell lines. However, treatment with the beta-adrenergic agonist isoprenaline
showed a concentration-dependent increase of the proliferation rate of both the UROtsa
and the T24 cell line. Further, isoprenaline gave a significantly higher increase
in proliferation rate in the UROtsa cell line compared to the T24 cell line (at 10−2 M, 367%±19% and 277%±20%, respectively; p=0.018; n=10 and 7). In both cell lines, this effect could be concentration-dependently antagonized
by the β3-selective adrenoceptor antagonist L-748,337, but not by the β1/β2-selective antagonist propranolol. Meanwhile, the α1-adrenoceptor agonist phenylephrine yielded a concentration-dependent decrease of
the proliferation rate of both cell lines (at 10−2 M, 22.0%±4.7% and 65.9%±4.8% in the UROtsa and T24 cell lines, respectively; p<0.0001; n=6).
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© 2010 Published by Elsevier Inc.
