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Autonomic receptor-mediated influence on the proliferation of the human bladder urothelial UROtsa and T24 cell lines

      Several reports have shown that changes in autonomic receptor expression during disease can influence the contractile response (1,2). The present study aimed to characterize how activation of autonomic receptors affects the proliferation rate of a normal and a malignant urothelial cell line, designated UROtsa and T24, respectively. Both cell lines were treated with a muscarinic, adrenergic or nicotinic receptor agonist. Neither the muscarinic agonist methacholine nor nicotine affected the proliferation rate of any of the two cell lines. However, treatment with the beta-adrenergic agonist isoprenaline showed a concentration-dependent increase of the proliferation rate of both the UROtsa and the T24 cell line. Further, isoprenaline gave a significantly higher increase in proliferation rate in the UROtsa cell line compared to the T24 cell line (at 10−2 M, 367%±19% and 277%±20%, respectively; p=0.018; n=10 and 7). In both cell lines, this effect could be concentration-dependently antagonized by the β3-selective adrenoceptor antagonist L-748,337, but not by the β12-selective antagonist propranolol. Meanwhile, the α1-adrenoceptor agonist phenylephrine yielded a concentration-dependent decrease of the proliferation rate of both cell lines (at 10−2 M, 22.0%±4.7% and 65.9%±4.8% in the UROtsa and T24 cell lines, respectively; p<0.0001; n=6).
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