Abstract
We previously showed that sympathectomy induces thickened intima and decreases the
expression of cytoskeletal proteins associated with a differentiated smooth muscle
cell (SMC) phenotype in hypercholesterolemic rats. In the present study, we sought
to determine the effect of sympathectomy on various components of the extracellular
matrix (ECM) in the aorta from these animals, since the state of SMC differentiation
depends on the nature of ECM components.
Collagen types I and III, previously reported to be associated with SMC dedifferentiation,
and collagen VI, elastin, laminin and elastin–laminin receptor (E/L-R), previously
reported to be associated with SMC differentiation, were analyzed by western immunoblot
and confocal microscopy in abdominal aortae from sham rats and hypercholesterolemic
rats sympathectomized with guanethidine.
Both western immunoblot and immunohistological analysis showed an increase in collagens
I and III (more for collagen I), with abundant labeling in the media, adventitia and
thickened intima in sympathectomized aortae. Collagen IV labeling was decreased in
the media and adventitia and was weak in the thickened intima in sympathectomised
aortae. The E/L-R increased and was abundantly labeled in the media and weakly in
the thickened intima in sympathectomized aortae. Elastin and laminin decreased and
appeared less labeled in the media in the sympathectomised aortae. In the thickened
intima, laminin was slightly labeled while elastin was not obviously labeled.
These data show that sympathectomy favors the ECM features reported in association
with a dedifferentiated/immature SMC phenotype and intimal thickening, probably by
actions on both SMCs and fibroblasts.
Keywords
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Article info
Publication history
Published online: August 08, 2011
Accepted:
July 11,
2011
Received in revised form:
July 10,
2011
Received:
November 17,
2010
Identification
Copyright
© 2011 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
