Abstract
We applied a microdialysis technique to the left ventricular myocardium of anesthetized
mice and tried to monitor acetylcholine (ACh) release from cardiac vagal nerves. Transection
of bilateral cervical vagal nerves decreased dialysate ACh concentration. Electrical
stimulation of the left cervical vagal nerve increased dialysate ACh concentration
in proportion to the frequency of stimulation. Intravenous administration of hexamethonium,
prevented the increase in dialysate ACh concentration during vagal nerve stimulation,
indicating that ACh in the dialysate primarily reflects ACh released from post-ganglionic
cardiac vagal nerves. Microdialysis permits monitoring of ACh release from post-ganglionic
cardiac vagal nerves that are most likely to be innervating the left ventricle in
mice.
Keywords
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Article info
Publication history
Published online: March 18, 2013
Accepted:
February 11,
2013
Received in revised form:
January 31,
2013
Received:
December 7,
2012
Identification
Copyright
© 2013 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
