Exercise training (ET) in treadmill running reduces oxidative stress and inflammatory signaling of the commissural nucleus of the solitary tract (commNTS) and rostral ventrolateral medulla in rats

      Background: Inflammation has been associated with cardiovascular diseases and the key point is the generation of ROS. Exercise can modulate medullary neurons involved in cardiovascular control. Aim: To evaluate the gene expression of antioxidant proteins against ROS and proteins involved in inflammatory process in commNTS and RVLM in rats submitted to ExT. Methods: Male Wistar rats (N = 7/group) were submitted to ExT in a treadmill running (1 h/day, 5 days/wk/10 wks) or maintained sedentary (Sed). Superoxide dismutase (SOD), catalase (CAT), neuroglobin (Ngb), Cytoglobin (Ctb), NADPH oxidase (Nox), cicloxigenase-2, and neuronal nitric oxide synthase (nNOS) gene expression was evaluated in commNTS and RVLM neurons by qPCR. Data are as mean ± EP (ΔΔCt comparative method, unpaired Student t-test, p < 0.05). Results: Gene expression of SOD in the commNTS (1.38 ± 0.13ExT vs. 0.90 ± 0.10Sed) and RVLM (1.43 ± 0.11ExT vs. 1.00 ± 0.08Sed) and CAT in the commNTS (1.31 ± 0.13ExT vs. 0.89 ± 0.10Sed) and RVLM (1.34 ± 0.09ExT vs. 1.00 ± 0.08Sed) increased in ExT compared to Sed. Expression of Ngb (1.29 ± 0.04ExT vs. 1.00 ± 0.07Sed) and Ctb (1.18 ± 0.02ExT vs. 1.02 ± 0.07Sed) increased in the RVLM in ExT. Expression of Nox decreased in the RVLM in ExT (0.55 ± 0.15 vs. 1.00 ± 0.08Sed). Cicloxigenase-2 reduced in the commNTS in ExT (0.39 ± 0.11 vs. 1.00 ± 0.15Sed) and RVLM in ExT (0.26 ± 002 vs. 0.97 ± 0.09Sed). Expression of nNOS diminished in ExT in the commNTS (0.15 ± 0.05 vs. 1.00 ± 0.62Sed) and RVLM (0.38 ± 0.08 vs. 0.97 ± 0.23Sed). Conclusion: Gene expression of proteins of the inflammatory process reduced while gene expression of antioxidant proteins increased in the commNTS and RVLM after ExT, suggesting a decrease in oxidative stress of downstream pathways mediated by nitric oxide. Financial support: FAPESP and NEPAS.
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