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Reduced colonic smooth muscle cholinergic responsiveness is associated with impaired bowel motility after chronic experimental high-level spinal cord injury

  • Author Footnotes
    1 Authors contributed equally.
    ,
    Author Footnotes
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    B. Frias
    Footnotes
    1 Authors contributed equally.
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    Affiliations
    International Collaboration on Repair Discoveries, University of British Columbia, Canada
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  • Author Footnotes
    1 Authors contributed equally.
    ,
    Author Footnotes
    3 Foothills Hospital Health Science Centre, Calgary, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
    A.A. Phillips
    Footnotes
    1 Authors contributed equally.
    3 Foothills Hospital Health Science Centre, Calgary, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
    Affiliations
    International Collaboration on Repair Discoveries, University of British Columbia, Canada

    Experimental Medicine Program, University of British Columbia, Canada

    Department of Physiology and Pharmacology, Hotchkiss Brain Institute, Libin Cardiovascular Institute, Cumming School of Medicine, University of Calgary, Canada
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  • Author Footnotes
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    J.W. Squair
    Footnotes
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    Affiliations
    International Collaboration on Repair Discoveries, University of British Columbia, Canada
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  • Author Footnotes
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    A.H.X. Lee
    Footnotes
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    Affiliations
    International Collaboration on Repair Discoveries, University of British Columbia, Canada

    Experimental Medicine Program, University of British Columbia, Canada
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  • Author Footnotes
    4 Department of Anesthesiology, Pharmacology & Therapeutics Faculty of Medicine University of British Columbia, 2176 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.
    I. Laher
    Footnotes
    4 Department of Anesthesiology, Pharmacology & Therapeutics Faculty of Medicine University of British Columbia, 2176 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.
    Affiliations
    Department of Anaesthesiology, Pharmacology and Therapeutics, University of British Columbia, Canada
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  • A.V. Krassioukov
    Correspondence
    Corresponding author at: ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    Affiliations
    International Collaboration on Repair Discoveries, University of British Columbia, Canada

    Experimental Medicine Program, University of British Columbia, Canada

    Division of Physical Medicine and Rehabilitation, Department of Medicine, University of British Columbia, Canada

    GF Strong Rehabilitation Center, Vancouver Coastal Health, Vancouver, BC, Canada
    Search for articles by this author
  • Author Footnotes
    1 Authors contributed equally.
    2 ICORD-BSCC, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada.
    3 Foothills Hospital Health Science Centre, Calgary, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
    4 Department of Anesthesiology, Pharmacology & Therapeutics Faculty of Medicine University of British Columbia, 2176 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.
Published:September 01, 2018DOI:https://doi.org/10.1016/j.autneu.2018.08.005
Reduced colonic smooth muscle cholinergic responsiveness is associated with impaired bowel motility after chronic experimental high-level spinal cord injury
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      Abstract

      The mechanisms underlying bowel dysfunction after high-level spinal cord injury (SCI) are poorly understood. However, impaired supraspinal sympathetic and parasympathetic control is likely a major contributing factor. Disruption of the descending autonomic pathways traversing the spinal cord was achieved by a T3 complete spinal cord transection, and colonic function was examined in vivo and ex vivo four weeks post-injury. Total gastrointestinal transit time (TGTT) was reduced and contractility of the proximal and distal colon was impaired due to reduced M3 receptor sensitivity. These data describe a clinically relevant model of bowel dysfunction after SCI.

      Keywords

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      Article info

      Publication history

      Published online: September 01, 2018
      Accepted: August 31, 2018
      Received in revised form: August 29, 2018
      Received: November 25, 2017

      Footnotes

      ☆Sources of funding: Killam Laureates (AAP, JWS); Heart and Stroke Foundation of Canada (AAP, AVK); MSFHR (AAP). Craig H. Neilson Foundation (AVK, AAP), Canadian Institute of Health Research, Canadian Foundation for Innovation, Paralyzed Veterans of America (AVK).

      Identification

      DOI: https://doi.org/10.1016/j.autneu.2018.08.005

      Copyright

      © 2018 Elsevier B.V. All rights reserved.

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