Highlights
- •ICV injected nesfatin-1 increases the immunoreactivities of the hypothalamic COXs and LOX.
- •Centrally administrated nesfatin-1 augments hypothalamic COXs and LOX protein expression
- •ICV injection of nesfatin-1 causes an increase in posterior hypothalamic extracellular total prostaglandin level.
Abstract
Nesfatin-1 is a multifunctional neuropeptide having crucial autonomic roles. It is
well known that nesfatin-1 collaborates with other central neuromodulatory systems,
such as central corticotropin-releasing hormone, melanocortin, oxytocin, and cholinergic
systems to show its autonomic effects. Central arachidonic acid cascade plays an important
role to provide the homeostasis by exhibiting similar autonomic effects to nesfatin-1.
Based on these similarities, the current study was designed to show the effects of
intracerebroventricularly (ICV) injected nesfatin-1 on the hypothalamic arachidonic
acid (AA) cascade.
Immunochemistry and western blot approaches demonstrated that ICV administration of
nesfatin-1 provokes an increase in the hypothalamic cyclooxygenase (COX) -1, -2 and
lipoxygenase (LOX) protein expression. Moreover, the microdialysis study demonstrated
that centrally injected nesfatin-1 increased the posterior hypothalamic extracellular
AA products.
In conclusion, these findings report that while nesfatin-1 is generating its autonomic
effects, it also might be using central prostaglandins and leukotrienes by activating
central COX and LOX pathways.
Keywords
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Article info
Publication history
Published online: April 16, 2020
Accepted:
March 23,
2020
Received in revised form:
March 19,
2020
Received:
January 9,
2020
Identification
Copyright
© 2020 Elsevier B.V. All rights reserved.
