Abstract
The immunohistochemical localization of proteins for synaptic release was examined
in smooth muscle-associated sensory nerve endings using whole-mount preparations of
the rat trachea. Plant-like smooth muscle-associated nerve endings with immunoreactivity
for Na+-K+-ATPase, α3-subunit were identified in the trachealis muscle. VGLUT1, synapsin1, t-SNARE proteins
(SNAP25 and syntaxin1), v-SNARE proteins (VAMP1 and VAMP2), and a presynaptic active
zone-related protein (piccolo) were detected in the terminal parts of these endings.
These results suggest that smooth muscle-associated nerve endings secrete glutamate
to modulate sensorimotor functions in the lung deflation reflex.
Keywords
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References
- NMDA receptors mediate contractile responses in human airway smooth muscle cells.Am. J. Physiol. Lung Cell. Mol. Physiol. 2015; 308: 1253-1264https://doi.org/10.1152/ajplung.00402.2014
- Glutamatergic modulation of synaptic-like vesicle recycling in mechanosensory lanceolate nerve terminals of mammalian hair follicles.J. Physiol. 2013; 591: 2523-2540https://doi.org/10.1113/jphysiol.2012.243659
- Autogenic modulation of mechanoreceptor excitability by glutamate release from synaptic-like vesicles: evidence from the rat muscle spindle primary sensory ending.J. Physiol. 2005; 562: 381-394https://doi.org/10.1113/jphysiol.2004.074799
- Sensory receptors in the airways: neurochemical coding of smooth muscle-associated airway receptors and pulmonary neuroepithelial body innervation.Auton. Neurosci. 2006; 126–127: 307-319https://doi.org/10.1016/j.autneu.2006.02.006
- Are unconventional NMDA receptors involved in slowly adapting type I mechanoreceptor responses?.Neuroscience. 2005; 133: 763-773https://doi.org/10.1016/j.neuroscience.2005.03.018
- The synapsins: key actors of synapse function and plasticity.Prog. Neurobiol. 2010; 91: 313-348https://doi.org/10.1016/j.pneurobio.2010.04.006
- What is the role of SNARE proteins in membrane fusion?.Am. J. Physiol. Cell Physiol. 2003; 285 (54-52)https://doi.org/10.1152/ajpcell.00091.2003
- Vesicular glutamate transporter immunoreactivity in the periodontal ligament of the rat incisor.Anat. Rec. 2012; 295: 160-166https://doi.org/10.1002/ar.21465
- Immunohistochemical localization of SNARE core proteins in intrapulpal and intradentinal nerve fibers of rat molar teeth.Arch. Oral Biol. 2017; 73: 248-252https://doi.org/10.1016/j.archoralbio.2016.10.025
- Sensory nerves in lung and airways.Compr. Physiol. 2014; 4: 287-324https://doi.org/10.1002/cphy.c130020
- Slowly adapting sensory units have more receptors in large airways than in small airways in rabbits.Front. Physiol. 2016; 7: 1-7https://doi.org/10.3389/fphys.2016.00588
- Dopamine secretion is mediated by sparse active zone-like release sites.Cell. 2018; 172e15https://doi.org/10.1016/j.cell.2018.01.008
- A single baroreceptor unit consists of multiple sensors.Sci. Rep. 2021; 11: 23111https://doi.org/10.1038/S41598-021-02563-X
- Vagal afferent innervation of the airways in health and disease.Physiol. Rev. 2016; 96: 975-1024https://doi.org/10.1152/physrev.00039.2015
- Sensory receptors in the visceral pleura: neurochemical coding and live staining in whole mounts.Am. J. Resp. Cell Mol. Biol. 2007; 36: 541-551https://doi.org/10.1165/rcmb.2006-0256OC
- Molecular organization of the presynaptic active zone.Cell Tissue Res. 2006; 326: 379-391https://doi.org/10.1007/S00441-006-0244-Y
- Morphology and chemical characteristics of subepithelial laminar nerve endings in the rat epiglottic mucosa.Histochem. Cell Biol. 2012; 138: 25-39https://doi.org/10.1007/s00418-012-0939-y
- Vesicle-released glutamate is necessary to maintain muscle spindle afferent excitability but not dynamic sensitivity in adult mice.J. Physiol. 2021; 599: 2953-2967https://doi.org/10.1113/JP281182
- Vesicular glutamate transporter immunoreactivity in the central and peripheral endings of muscle-spindle afferents.Brain Res. 2004; 1011: 247-251https://doi.org/10.1016/j.brainres.2004.03.047
- Vagal afferent nerve endings in the trachealis muscle of the dog.Arch. Histol. Cytol. 1994; 57: 473-480
- Calretinin immunoreactive nerve endings in the trachea and bronchi of the rat.J. Vet. Med. Sci. 1999; 61: 267-269
- Immunohistochemical distribution of proteins involved in glutamate release in subepithelial sensory nerve endings of rat epiglottis.Histochem. Cell Biol. 2022; 157: 51-63https://doi.org/10.1007/S00418-021-02038-0
- Vesicular glutamate transporter 2-immunoreactive afferent nerve terminals in the carotid body of the rat.Cell Tissue Res. 2014; 358: 271-275https://doi.org/10.1007/s00441-014-1921-x
- Vesicular glutamate transporter 2-immunoreactive afferent nerve terminals in rat carotid sinus baroreceptors.Acta Histochem. 2020; 122151469https://doi.org/10.1016/j.acthis.2019.151469
- Structure of slowly adapting pulmonary stretch receptors in the lung periphery.J. Appl. Physiol. 2003; 95: 385-393https://doi.org/10.1152/japplphysiol.00137.2003
Article info
Publication history
Published online: November 07, 2022
Accepted:
November 3,
2022
Received in revised form:
October 8,
2022
Received:
July 30,
2022
Identification
Copyright
© 2022 Elsevier B.V. All rights reserved.
