Abstract
Background
The superior cervical ganglion (SCG) plays critical roles in the regulation of blood
pressure and cardiac output. Metabotropic glutamate receptors (mGluRs) in the SCG
are not clearly elucidated yet. Most studies on the expression and functions of mGluRs
in the SCG focused on the cultured SCG neurons, and yet little information has been
reported in the SCG tissue. Chronic intermittent hypoxia (CIH), one of the major clinical
features of obstructive sleep apnea (OSA) patients, is a critical pathological cause
of secondary hypertension in OSA patients, but its impact on the level of mGluRs in
the SCG is unknown.
Objective
To explore the expression and localization of mGluR2/3 and the effect of CIH on mGluR2/3
level in rat SCG tissue.
Methods
RT-PCR and immunostaining were conducted to examine the mRNA and protein expression
of mGluR2/3 in rat SCG. Immunofluorescence staining was conducted to examine the distribution
of mGluR2/3. Rats were divided into control and CIH group which the rats were exposed
to CIH for 6 weeks. Western blots were performed to examine the level of mGluR2/3
in rat SCG.
Results
mRNAs of mGluR2/3 expressed in rat SCG. mGluR2 distributed in principal neurons and
small intensely fluorescent cells but not in satellite glial cells, nerve fibers,
and vascular endothelial cells; mGluR3 was detected in nerve fibers rather than in
the cells mentioned above. CIH exposure reduced the protein level of mGluR2/3 in rat
SCG.
Conclusion
mGluR2/3 exists in rat SCG with diverse distribution patterns, and may be involved
in CIH-induced hypertension.
Keywords
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Article info
Publication history
Published online: November 21, 2022
Accepted:
November 17,
2022
Received in revised form:
October 6,
2022
Received:
June 28,
2022
Identification
Copyright
© 2022 Elsevier B.V. All rights reserved.
