Abstract
Substance-P (SP) is a commonly used marker of nociceptive afferent axons, and it plays an important
role in a variety of physiological functions including the regulation of motility,
gut secretion, and vascular flow. Previously, we found that SP-immunoreactive (SP-IR)
axons densely innervated the pyloric antrum of the flat-mount of the mouse whole stomach
muscular layer. However, the regional distribution and morphology of SP-IR axons in
the submucosa and mucosa were not well documented. In this study, the mouse antrum-pylorus-duodenum
(APD) were transversely and longitudinally sectioned. A Zeiss M2 imager was used to
scan the serial sections of each APD (each section montage consisted of 50–100 all-in-focus
maximal projection images). To determine the detailed structures of SP-IR axons and
terminals, we used the confocal microscope to scan the regions of interest. We found
that 1) SP-IR axons innervated the muscular, submucosal, and mucosal layers. 2) In
the muscular layer, SP-IR varicose axons densely innervated the muscles and formed
varicose terminals which encircled myenteric neurons. 3) In the submucosa, SP-IR axons
innervated blood vessels and submucosal ganglia and formed a network in Brunner’s
glands. 4) In the mucosa, SP-IR axons innervated the muscularis mucosae. Some SP-IR
axons entered the lamina propria. 5) The muscular layer of the antrum and duodenum
showed a higher SP-IR axon density than the pyloric sphincter. 6) SP-IR axons were
from extrinsic and intrinsic origins. This work provided a comprehensive view of the
distribution and morphology of SP-IR axons in the APD at single cell/axon/varicosity
scale. This data will be used to create a 3D scaffold of the SP-IR axon innervation
of the APD.
Keywords
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Article info
Publication history
Published online: January 27, 2023
Accepted:
January 24,
2023
Received in revised form:
January 17,
2023
Received:
June 29,
2022
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© 2023 Published by Elsevier B.V.
